Rib histomorphometry: A reliability and validation study with a critical review of histological techniques for forensic age estimation

Abstract Fragmented human remains present a challenge for forensic experts as they attempt to identify individuals using standard methods. Several histological age estimation techniques have been developed during the last fifty years aid in this process. However, very few validation studies conducted order test their accuracy and bias, thus, assessment is required we employ them while testifying court. Histological variables are assessed from rib thin sections two Mediterranean samples; Cretans (N = 41) Greek-Cypriots (N = 47). Intra inter-observer errors through TEM analysis Intra-class Correlation Coefficient by testing observers with different levels of experience collected data on osteon counts area measurements. The relation between determined correlation coefficients. Histomorphometric applied four widely used formulas assessing performance methods entire sample. Inaccuracy bias calculated estimations known tested significance proportional assessed. Overall, parameters presented acceptable intra- errors. All exhibited statistically significant (P  This study explores inter-population variation bone remodeling dynamics presents critical evaluation methodological issues that can affect existing techniques.